Mechanistic Characterization of Alternating Current Cloud Point Extraction in a Microchannel: Extraction under Physiological Temperature
نویسندگان
چکیده
We present mechanistic characterization of alternating current cloud point extraction (ACPE) in a microchannel. ACPE is our original technique for on-chip extraction and preconcentration of membrane-associated biomolecules [1]. Joule heating of nonionic surfactant micellar solutions plays an important role in ACPE, but temperature of solutions during the extraction has not been investigated. In this study, we estimated the temperature under various experimental conditions using a temperature-dependent fluorescent dye. The ACPE was achieved around 36°C, which is physiological temperature, under optimized conditions. These results provide guidelines for use of the ACPE technique in microfluidic chemical and biochemical analyses.
منابع مشابه
Simultaneous determination of Tropaeolin O and brilliant blue in food samples after cloud point extraction
In this study, a simple and low-cost method was developed for extraction and pre-concentration of brilliant blue(BB) and Tropaeolin O(TO) in food samples using cloud point extraction (CPE) prior to spectrophotometric determination. The effects of main factors such as pH, surfactant and salt concentrations, incubation time and temperature on the cloud point extraction of both dyes were investiga...
متن کاملAlternating current cloud point extraction on a microchip for preconcentration of membrane-associated biomolecules.
Joule heating and negative dielectrophoresis, caused by AC electric fields in a microchannel, have been employed for cloud point extraction of phospholipid as a model of membrane-associated biomolecules.
متن کاملSimultaneous Determination of Carbazoles in Water Samples by Cloud Point Extraction Coupled to HPLC
Cloud point extraction (CPE) as a rapid, simple and efficient method coupled with high performance liquid chromatography (HPLC) was used for sample preparation and subsequent determination of carbazole, trinitrocarbazole (TrNC) and tetra nitro carbazole (TNC) in water samples. Some effective parameters on extraction, such as volume of Triton X-100, extraction time, extraction temperature, ionic...
متن کاملSeparation, Preconcentration and determination of Hg (II) Ion in Water Samples by Cloud Point Extraction Technique Coupled with UV-VIS Spectrophotometry using a New Complexing Agent
In this study a cloud-point extraction process using non-ionic surfactant Triton X-114 for extraction of mercury (II) from aqueous solutions was developed. The method is based on the complex formation of Hg (II) with benzyl (pyridin-2-yl) methylcarbamodithioate at pH 5.5. After preconcentration and dilution of the surfactant-rich phase, the enriched analyte was determined by s...
متن کاملThe Effect of Type and Concentration of Surfactant and Ligand on Uranium (VI) Cloud-Point Extraction (CPE) from Aqueous Solutions (Short Communication)
In this article cloud-point extraction (CPE) was used with chelating agent to extract uranium from aqueous solutions. The methodology used is based on the formation of metal complexes soluble in a micellar phase of surfactant. The metal ions complexes are then extracted into the surfactant-rich phase at a temperature above the cloud-point temperature. The effect of type of surfactants and ligan...
متن کامل